Intermediate

Part:BBa_S04147

Designed by: Tom Tran   Group: Guests   (2008-10-28)

The Partial Butanol Operon (5 inserts from I725021 to I725025)

The partial butanol operon required for Clostridium acetobutylicum to convert acetyl-CoA to butanol. The operon includes the following butanol inserts:

  1. RBS + B-hydroxy butyryl CoA dehydrogenase
  2. RBS + Enoyl-CoA hydratase
  3. RBS + Butyryl CoA dehydrogenase
  4. RBS + Butyraldehyde dehydrogenase
  5. RBS + Butanol dehydrogenase


For the complete butanoate pathway to operate optimally, the following inserts are required in addition to this operon:

  • ETFA (electron-transfer-flavoprotein, alpha polypeptide)
  • ETFB (electron-transfer-flavoprotein, beta polypeptide)
  • Thiolase


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 763
    Illegal AgeI site found at 1077
    Illegal AgeI site found at 1208
    Illegal AgeI site found at 1988
    Illegal AgeI site found at 3091
    Illegal AgeI site found at 3493
    Illegal AgeI site found at 4717
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None